The antioxidant and Hormonal Effects of Ginseng Panax on Lifespan and Stres Resistance in Caenorhabditis elegans

Abstract

The Thesis: The Antioxidant and Hormonal Effects of Ginseng Panax on Lifespan and Stress Resistance in Caenorhabditis elegans Made by: Václav Ježek The antioxidant and hormonal effect of Panax ginseng on lifespan and stress resistance in C. elegans were investigated. The best results by treatment of C. elegans were achieved in the presence of pure GPE 50 µg/ml without the presence of cholesterol. Surprisingly this medium assigns the increase of mean survival by 10.98 % in contrasting with medium containing the mixture of GPE 50 µg/ml and cholesterol. This negative impact of GPE and cholesterol co- administration on lifespan of C. elegans is more obvious by using sixfold higher concentration of GPE (300 µg/ml). In this case the increase of mean survival by treatment with pure GPE is 17.69 %. In addition the highest increase of progeny was observed in medium with pure GPE 300 µg/ml. Surprisingly this concentration was appointed as toxic with negative effect on lifespan of C. elegans. It is obvious that GPE does not protect C. elegans against oxidative stress significantly because it does not suppress the expression of hsp-16.2/GFP and does not protect against oxidative stress caused by lethal dose of juglone. These findings suggest that the antioxidant activity of GPE does not play prevailing role by...

The Thesis: The Antioxidant and Hormonal Effects of Ginseng Panax on Lifespan and Stress Resistance in Caenorhabditis elegans Made by: Václav Ježek The antioxidant and hormonal effect of Panax ginseng on lifespan and stress resistance in C. elegans were investigated. The best results by treatment of C. elegans were achieved in the presence of pure GPE 50 µg/ml without the presence of cholesterol. Surprisingly this medium assigns the increase of mean survival by 10.98 % in contrasting with medium containing the mixture of GPE 50 µg/ml and cholesterol. This negative impact of GPE and cholesterol co- administration on lifespan of C. elegans is more obvious by using sixfold higher concentration of GPE (300 µg/ml). In this case the increase of mean survival by treatment with pure GPE is 17.69 %. In addition the highest increase of progeny was observed in medium with pure GPE 300 µg/ml. Surprisingly this concentration was appointed as toxic with negative effect on lifespan of C. elegans. It is obvious that GPE does not protect C. elegans against oxidative stress significantly because it does not suppress the expression of hsp-16.2/GFP and does not protect against oxidative stress caused by lethal dose of juglone. These findings suggest that the antioxidant activity of GPE does not play prevailing role by...